Figure 2From: Overexpressed HDGF as an independent prognostic factor is involved in poor prognosis in Chinese patients with liver cancerHDGF was highly expressed in HepG2 cells and this level was successfully knocked down by shRNA. A. Total RNA was extracted from HepG2 cells and RT-PCR was applied to examine the steady-state mRNA levels of HDGF and GAPDH. B and C. Parental HepG2 cells and those stably transfected with either control (shCtrl) or HDGF-specific (shHDGF) shRNA were examined for HDGF expression by quantitative real-time PCR and Western-blot. GAPDH and α-tubulin were used as internal control, respectively.Back to article page