Table 1 Weigert-Lead stain (M. Jondet)
Staining solutions | |
|---|---|
1 - Weigert's iron haematoxylin | |
a - Haematoxylin solution | |
Haematoxylin | 1 g |
Ethanol | 100 ml |
b - Iron solution | |
30% Ferric chloride solution in water | 4 ml |
Hydrochloric acid 1 M | 1 ml |
Distilled water | 95 ml |
Mix equal volumes of solution a and b, use within 60 min | |
2 - Lead citrate | |
lead nitrate | 1.33 g |
sodium citrate | 2.10 g |
distilled water | 30 ml |
Add sodium hydroxyde 0,4 M untill precipitate dissolves | |
Method | |
a - dewax and rehydrate sections | |
b - Weighert's haematoxylin | 1 minute |
c - wash with distilled water | |
d - saturated picric acid in water | 1 minute |
e - wash with distilled water | |
f - lead citrate | 1 minute |
g - wash with water | |
h - mount in Eukitt after alcohol and xylene clarification | |
Results | |
Nucleus | Black |
Nuclear membrane | dark black |
Cytoplasm | light grey |