Table 1 Specification of Primers used in RT-PCR and qRT-PCR Experiments
S. No. | Target Gene | Primer Name | Primer Sequence 5'-----------------3' |
|---|---|---|---|
1) | EBV encoded RNA 1 (EBER1) | EB1 (Forward) EB1 (Reverse) | 5'- AGGACCTACGCTGCCCTAGA -3' 5'- AAAACATGCGGACCACCAGCTGG -3' |
2) | EBV encoded RNA 2 (EBER2) | EB2 (Forward) EB2 (Reverse) | 5'- AGGACAGCCGTTGCCCTAGTG -3' 5'- TAGCGGACAAGCCGAATACCCT -3' |
3) | Interleukin 10 (IL10), mRNA | IL-10 (Forward) IL-10 (Reverse) | 5'- ATGCACAGCTCAGCACTGCTCTG -3' 5'- GGAAGAAATCGATGACAGCGCCG -3' |
4) | Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), mRNA | GAP (Forward) GAP (Reverse) | 5'- CAAGGTCATCCATGACAACTTTG -3' 5'- GTCCACCACCCTGTTGCTGTAG -3' |