Bubble plot display of interrelationship between the HER2 membrane connectivity estimate and HER2 FISH data. Horizontal axis represents mean CEP17 by FISH analysis. Left vertical axis represents mean HER2 by FISH analysis. Right vertical axis represents HER2/CEP17 ratio. Bubble size is proportional to the patient's maximum HER2 connectivity value. Black dashed vertical reference line separates cases with polysomy (CEP17 > 3) to the right. Blue and orange dashed horizontal reference lines separate cases with amplification (HER2 > 6 and HER2/CEP17 > 2) above. Each patient is represented by two bubbles with the same CEP17 value: blue bubble maps to the mean HER2 orange bubble - to the mean HER2/CEP17 ratio.