Table 2 Primers used for PCR
From: Apoptosis signal-regulating kinase 1 is associated with the effect of claudin-6 in breast cancer
Gene | Sequence of primers | Size of PCR(bp) | Denaturation temperature (°C) | Cycles | |
|---|---|---|---|---|---|
ASK1 | sense | 5’-TTCACACAAAACGGATGTAACATT-3’ | 198 | 56 | 30 |
antisense | 5’-CCTAAACAGTTATGGTCACATTTTGG-3’ | ||||
Claudin-6 | sense | 5’-TTCATCGGCAACAGCATCGT-3’ | 345 | 58 | 35 |
antisense | 5’-GGTTATAGAAGTCCCGGATGA-3’ | ||||
GAPDH | sense | 5’-TGTTGCCATCAATGACCCCTT-3’ | 202 | 56 | 25 |
|  | antisense | 5’-CTCCACGACGTACTCAGCG-3’ |  |  |  |