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Table 2 Primers used for PCR

From: Apoptosis signal-regulating kinase 1 is associated with the effect of claudin-6 in breast cancer

Gene Sequence of primers Size of PCR(bp) Denaturation temperature (°C) Cycles
ASK1 sense 5’-TTCACACAAAACGGATGTAACATT-3’ 198 56 30
antisense 5’-CCTAAACAGTTATGGTCACATTTTGG-3’
Claudin-6 sense 5’-TTCATCGGCAACAGCATCGT-3’ 345 58 35
antisense 5’-GGTTATAGAAGTCCCGGATGA-3’
GAPDH sense 5’-TGTTGCCATCAATGACCCCTT-3’ 202 56 25
  antisense 5’-CTCCACGACGTACTCAGCG-3’