Table 1 Targeted Mutations and Sequences for EGFR, KRAS and BRAF mutations
From: Pyrosequencing data analysis software: a useful tool for EGFR, KRAS, and BRAF mutation analysis
Targeted Mutations | Targeted Sequences |
|---|---|
EGFR exon 18, codon 719 | DSCTCCGGTGC |
EGFR exon 19 deletions | TATCAA[GGAATTAAGAGAAGC]AACATCTCCGAAAGCCA |
EGFR exon 20, Codon 768 | CAGCGTGGACAACCCCCACG |
EGFR exon 20, Codon 790 | ATCAYG |
EGFR exon 21 | CKGGCCAAACDGCTGGGT |
KRAS codon 12 & 13 | NNTGRCGTAGGC |
KRAS codon 61 (reverse sequencing) | CTCDTGACCTG |
BRAF codon 600 (reverse sequencing) | CWCTGTAG |