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Table 6 Gene-protein BISH (comparator) vs. single BISH (reference) agreement analyses

From: A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections

Single BISH
  Gene-protein BISH Amplified Non-amplified Total
Reader A Amplified 43 2 45
  Non-amplified 2 118 120
  Total 45 120 165
  PPA: n/N (%) (95% CI) 43/45 (95.6) (85.2–98.8)   
  NPA: n/N (%) (95% CI) 118/120 (98.3) (94.1–99.5)   
  OPA: n/N (%) (95% CI) 161/165 (97.6) (93.9–99.1)   
  Kappa coefficient (95% CI) 0.94 (0.88–1.00)   
Reader B Amplified 47 1 48
  Non-amplified 3 121 124
  Total 50 122 172
  PPA: n/N (%) (95% CI) 47/50 (94.0) (83.8–97.9)   
  NPA: n/N (%) (95% CI) 121/122 (99.2) (95.5–99.9)   
  OPA: n/N (%) (95% CI) 168/172 (97.7) (94.2–99.1)   
  Kappa coefficient (95% CI) 0.94 (0.89–1.00)   
Reader C Amplified 41 5 46
  Non-amplified 2 125 127
  Total 43 130 173
  PPA: n/N (%) (95% CI) 41/43 (95.3) (84.5–98.7)   
  NPA: n/N (%) (95% CI) 125/130 (96.2) (91.3–98.3)   
  OPA: n/N (%) (95% CI) 166/173 (96.0) (91.9–98.0)   
  Kappa coefficient (95% CI) 0.89 (0.82–0.97)   
  1. BISH: brightfield in situ hybridization; PPA: positive percent agreement; NPA: negative percent agreement; OPA: overall percent agreement.