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Table 8 Summary of inter-reader agreement analyses

From: A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections

Assay Minimum APA Maximum APA Minimum ANA Maximum ANA
Gene-protein IHC 95.2% 97.2% 98.1% 98.8%
Single IHC 92.2% 96.2% 97.0% 98.5%
Gene-protein BISH 93.5% 97.9% 97.7% 99.2%
Single BISH 88.2% 93.8% 95.5% 97.5%
Gene-protein IHC/BISH 91.7% 95.2% 96.4% 98.0%
Single IHC/BISH 89.7% 94.3% 95.2% 97.3%
  1. APA: average positive agreement; ANA: average negative agreement; IHC: immunohistochemistry; BISH: brightfield in situ hybridization.