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Table 8 Summary of inter-reader agreement analyses

From: A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections

Assay

Minimum APA

Maximum APA

Minimum ANA

Maximum ANA

Gene-protein IHC

95.2%

97.2%

98.1%

98.8%

Single IHC

92.2%

96.2%

97.0%

98.5%

Gene-protein BISH

93.5%

97.9%

97.7%

99.2%

Single BISH

88.2%

93.8%

95.5%

97.5%

Gene-protein IHC/BISH

91.7%

95.2%

96.4%

98.0%

Single IHC/BISH

89.7%

94.3%

95.2%

97.3%

  1. APA: average positive agreement; ANA: average negative agreement; IHC: immunohistochemistry; BISH: brightfield in situ hybridization.