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Table 9 Inter-reader agreement by reader pair and assay

From: A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections

Reader Pair

Assay

APA

ANA

A vs. B

Gene-protein IHC

96.1

98.5

 

Single IHC

93.9

97.8

 

Gene-protein BISH

97.9

99.2

 

Single BISH

93.8

97.5

 

Gene-protein IHC/BISH

95.2

98.0

 

Single IHC/BISH

93.3

96.9

A vs. C

Gene-protein IHC

97.2

98.8

 

Single IHC

96.2

98.5

 

Gene-protein ISH

93.5

97.7

 

Single BISH

92.1

97.0

 

Gene-protein IHC/BISH

94.4

97.6

 

Single IHC/BISH

94.3

97.3

B vs. C

Gene-protein IHC

95.2

98.1

 

Single IHC

92.2

97.0

 

Gene-protein BISH

93.8

97.7

 

Single BISH

88.2

95.5

 

Gene-protein IHC/BISH

91.7

96.4

 

Single IHC/BISH

89.7

95.2

  1. APA: average positive agreement; ANA: average negative agreement; IHC: immunohistochemistry; BISH: brightfield in situ hybridization.