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Table 9 Inter-reader agreement by reader pair and assay

From: A gene-protein assay for human epidermal growth factor receptor 2 (HER2): brightfield tricolor visualization of HER2 protein, the HER2 gene, and chromosome 17 centromere (CEN17) in formalin-fixed, paraffin-embedded breast cancer tissue sections

Reader Pair Assay APA ANA
A vs. B Gene-protein IHC 96.1 98.5
  Single IHC 93.9 97.8
  Gene-protein BISH 97.9 99.2
  Single BISH 93.8 97.5
  Gene-protein IHC/BISH 95.2 98.0
  Single IHC/BISH 93.3 96.9
A vs. C Gene-protein IHC 97.2 98.8
  Single IHC 96.2 98.5
  Gene-protein ISH 93.5 97.7
  Single BISH 92.1 97.0
  Gene-protein IHC/BISH 94.4 97.6
  Single IHC/BISH 94.3 97.3
B vs. C Gene-protein IHC 95.2 98.1
  Single IHC 92.2 97.0
  Gene-protein BISH 93.8 97.7
  Single BISH 88.2 95.5
  Gene-protein IHC/BISH 91.7 96.4
  Single IHC/BISH 89.7 95.2
  1. APA: average positive agreement; ANA: average negative agreement; IHC: immunohistochemistry; BISH: brightfield in situ hybridization.