Table 2 Applied antibodies and conditions for primary immunohistochemical reaction
From: N-cadherin is differentially expressed in histological subtypes of papillary renal cell carcinoma
| Â | N-Cadherin | E-Cadherin | P-Cadherin | KSP-Cadherin |
|---|---|---|---|---|
Clone | 6 G11 (mouse) | NCH-38 (mouse) | 56C1 (mouse) | MRQ-33 (mouse) |
Source | Dako, Hamburg | Dako, Hamburg | Linaris, Dossenheim | Zytomed Systems, Berlin |
| Â | Germany | Germany | Germany | Germany |
Pretreatment | citrate buffer | citrate buffer | citrate buffer | citrate buffer |
| Â | pH6.0 40 min | pH6.0 40 min | pH6.0 40 min | pH6.0 40 min |
Dilution and | 1:50 30 min RT | 1:50 30 min RT | 1:100 30 min RT | 1:50 30 min RT |
Incubation | Â | Â | Â | Â |