Molecular characterization of Listeria monocytogenes isolated from fresh seafood samples in Iran

Diagnostic Pathology

Table 1 Primers for amplification of virulence associated genes, Listeria spp. and serotypes of L. monocytogenes

Primer name	Primer sequence (5′-3′)	Target	Size of product (bp)	References
Lis1B	TTATACGCGACCGAAGCCAAC	L. innocua	870	[26]
Ino2	ACTAGCACTCCAGTTGTTAAAC
Lis1B	TTATACGCGACCGAAGCCAAC	L.	660	[26]
MonoA	CAAACTGCTAACACAGCTACT	monocytogenes
Lis1B	TTATACGCGACCGAAGCCAAC	L. ivanovii	1100	[26]
Iva1	CTACTCAAGCGCAAGCGGCAC
Lis1B	TTATACGCGACCGAAGCCAAC	L. seeligeri	1100	[26]
Sel1	TACACAAGCGGCTCCTGCTCAAC
Lis1B	TTATACGCGACCGAAGCCAAC	L. welshimeri	1050	[26]
Wel1	CCCTACTGCTCCAAAAGCAGCG
Lis1B	TTATACGCGACCGAAGCCAAC	L. grayi	480	[26]
MuraI	GTGATTTCTGCTTGCCATAG
prsF	GCTGAAGAGATTGCGAAAGAAG	All L. monocytogenes serovares	370	[27]
prsR	CAAAGAAACCTTGGATTTGCGG
lmo0737F	AGGGCTTCAAGGACTTACCC	L. monocytogenes serovar1/2a	691	[27]
lmo0737R	ACGATTTCTGCTTGCCATTC
ORF2819F	AGCAAAATGCCAAAACTCGT	L. monocytogenes serovar1/2b	471	[27]
ORF2819R	CATCACTAAAGCCTCCCATTG
ORF2110F	AGTGGACAATTGATTGGTGAA	L. monocytogenes serovar 4b	597	[27]
ORF2110R	CATCCATCCCTTACTTTGGAC
plc A-F	CTGCTTGAGCGTTCATGTCTCCATCCCCC	plcA gene	1484	[28]
plc A-R	CATGGGTTTCACTCTCCTTCTAC
prf A-F	CTGTTGGAGCTCTTCTTGGTGAAGCAATCG	prfA gene	1060	[28]
prf A-R	AGCAACCTCGGTACCATATACTAACTC
act A-F	CGCCGCGGAAATTAAAAAAAGA	actA gene	839	[29]
act A-R	ACGAAGGAACCGGGCTGCTAG
hly A-F	GCAGTTGCAAGCGCTTGGAGTGAA	hlyA gene	456	[30]
hly A-R	GCAACGTATCCTCCAGAGTGATCG
Iap-F	ACAAGCTGCACCTGTTGCAG	Iap gene	131	[31]
Iap-R	TGACAGCGTGTGTAGTAGCA

ISSN: 1746-1596