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Figure 2 | Diagnostic Pathology

Figure 2

From: Impaired binding of standard initiation factors eIF3b, eIF4G and eIF4B to domain V of the live-attenuated coxsackievirus B3 Sabin3-like IRES - alternatives for 5′UTR-related cardiovirulence mechanisms

Figure 2

Autoradiogram of an UV-crosslink assay performed with IRES domain V labeled probes and an extract prepared from HeLa cells. Proteins were resolved in a 10% SDS-PAGE. Domain V of FMDV IRES was used as positive control (lane DV FMDV). Lanes DV CVB3 (Wt) and DV CVB3 (S3) demonstrate the RNA–protein interaction pattern of CVB3 wild-type and Sabin3-like IRES domains V. Equal amounts of total proteins (10 μg) were loaded per well. The molecular weight (MW) of each complex was determined by comparing to a concurrently loaded MW marker: Prestained SDS-page standards broad range (Biorad).

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Diagnostic Pathology

ISSN: 1746-1596

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