Immunohistochemistry of E-cadherin, Vimentin and GP73 in PCL tissues and HCC tissue (400×). E-cadherin was detected in the cell membrane. Note that E-cadherin showed moderate and strong staining in s (PCL) (1) By contrast, it was almost absent in normal control HCC(2) (×400).Vimentin was detected in the cytoplasm and interstitial cells. Note that Vimentin was almost absent in normal controls (PCL) (3) By contrast, moderate and strong staining was seen in HCC (4) (×400).GP73 protein expression was evaluated by an En-Vision immunohistochemical method. Note that GP73 was almost absent in normal controls (PCL) (5). By contrast, moderate and strong staining of GP73 was identified in HCC, respectively. (×400). (6).