Immunohistochemical staining of Fascin in sciatic nerves of EAN rats. In normal rat sciatic nerves, Fascin expression was only occasionally observed in single cells (A). At day 7, the Fascin+ cells were observed clearly (B). Significant accumulation of Fascin+ cells could be observed at day 11 (C), maximal accumulation of Fascin+ cells in sciatic nerves was found at day 15 (D). From day 17, the accumulation of Fascin+ cells began to decrease (E), but finally the cellular accumulation returned to a control level by day 22 (F). Double staining experiments revealed that few Fascin+ cells co-expressed cd-3 (blue) representing T cells (G), and few Fascin+ cells co-expressed active macrophage marker ED1 (blue)at day 15 (H). Scale bars are 25 mm for all pictures.