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Table 1 Digital image analysis outputs and pathologist’s IHC HER2 score on the IHC multi-block tissue controls

From: Digital immunohistochemistry: new horizons and practical solutions in breast cancer pathology

  Core #1 HER2 0 Core #2 HER2 1+ Core #3 HER2 2+ Core #4 HER2 3+ Cores #1-4 Mean
Mean membrane intensity* 176 ± 16 190 ± 7 155 ± 11 86 ± 11 152 ± 8
% of cells with complete membranous staining* 1 ± 4 2 ± 5 17 ± 6 59 ± 6 19 ± 4
Total number of cells* 4277 ± 3205 6759 ± 3342 17536 ± 3382 19803 ± 4269 12094 ± 2778
Pathologist’s HER2 staining intensity score corresponding the expected category** 87/88 73/89 88/91 84/87 N/A
  1. * Mean ± standard deviation
  2. ** Data represented as a ratio of number of corresponding HER2 score/all cores evaluated. In all discordant cases, except in the category of Core#1 HER2 0, pathologist gave a lower intensity score than that expected for the category. Total number of IHC control slides evaluated was 91, except some tissue cores were considered inadequate by the pathologist (number of cores represented in the denominator).