Figure 1

EGFR (epidermal growth factor receptor) gene status in adrenocortical neoplasms: (a) This image demonstrates strong membrane EGFR expression (3+) in adrenocortical carcinoma, as detected by immunohistochemistry. (b) This image demonstrates an absence of membrane EGFR expression in adrenocortical adenoma, as detected by immunohistochemistry. (c) The cancer cells demonstrate high polysomy on chromosome 7 in adrenocortical carcinoma, as detected by FISH(fluorescence in situ hybridization). (d) The tumor cells display disomy for the EGFR in adrenocortical adenoma, as detected by FISH (Green signals represent the chromosome 7 centromere, and red signals represent the EGFR gene).