Table 3 Diagnostic values of BRAF mutation and various DNA methylation markers testing on FNAB specimens for PTC detection [percent (PTCs with BRAF mutation or methylation-positive/total PTCs, sensitivity; benign nodules with BRAF mutation or methylation-negative/total benign nodules, specificity; PTCs with BRAF mutation or methylation-positive/total positive cases, PPV; benign nodules with BRAF mutation or methylation-negative/total negative nodules, NPV)]
Genes | Cut-off values | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | Accuracy (%) |
|---|---|---|---|---|---|---|
BRAF V600E | N/A | 71.43 (30/42) | 100 (37/37) | 100 (30/30) | 75.51 (37/49) | 71.43 |
CALCA | 2.405 | 33.33 (14/42) | 81.08 (30/37) | 66.67 (14/21) | 51.72 (30/58) | 14.41 |
DAPK1 | 0.003 | 66.67 (28/42) | 83.78 (31/37) | 82.35 (28/34) | 68.89 (31/45) | 50.45 |
TIMP3 | 1.038 | 30.95 (13/42) | 75.68 (28/37) | 59.09 (13/22) | 49.12 (28/ 57) | 6.63 |
RAR-beta | 1.606 | 30.95 (13/42) | 75.68 (28/37) | 59.09 (13/22) | 49.12 (28/57) | 6.63 |
RASSF1A | 5.311 | 61.90 (26/42) | 78.38 (29/37) | 76.47 (26/34) | 62.22 (28/45) | 40.28 |
All five genes | N/A | 88.10 (37/42) | 75.68 (28/37) | 80.43 (37/46) | 84.85 (28/33) | 63.69 |