Diagnostic application of PIK3CA mutation analysis in Chinese esophageal cancer patients

Diagnostic Pathology

Table 2 Comparison of mutation frequencies of the PIK3CA oncogene detected by three testing methods

ME-Liquidchip vs Sanger sequencing				ME-Liquidchip vs Pyrosequencing				Sanger sequencing vs Pyrosequencing
ME-Liquidchip				ME-Liquidchip				Sanger sequencing
Sanger sequencing	WT	Mut	Total	Pyrosequencing	WT	Mut	Total	Pyrosequencing	WT	Mut	Total
WT	94	5	99	WT	94	6	100	WT	99	1	100
Mut	0	7	7	Mut	0	6	6	Mut	0	6	6
Total	94	12	106	Total	94	12	106	Total	99	7	106

The McNemar Test, ME-Liquidchip vs Sanger sequencing, ME-Liquidchip vs Prosequencing, Sanger sequencing vs Prosequencing were, respectively, 5.25 (p < 0.025), 6.36 (p < 0.025) and 1.01 (p > 0.25).
Wt, wild type; Mut, mutant.

ISSN: 1746-1596