Figure 3

miR-337 reduced cell invasion in vitro. The effects of miR-337 mimics (or NC) on the proliferation of PANC-1 and As-PC-1 cells in vitro were detected by wound healing assay and transwell invasion assay. A) PANC-1 and As-PC-1 cells at 12 h post-transfection were seeded in six-well plates and cultured for 24 h. Confluent monolayer cells were scratched with a 200 μl pipette tip and were allowed to close the wound for 36 h. Wound healing assays show the rate of invasion of PANC-1 (or As-PC-1) cells transfected with miR-337 mimics at 36 h were markedly lower than in the control groups (blank control and cells transfected with miR-337 NC). B) The number of cells that migrate through the Matrigel into the lower surface of the polycarbonic membrane was determined in a transwell invasion assay. Cell invasion was significantly less pronounced in miR-337-mimic-treated cells than in the blank control and NC groups. Asterisks indicate significance at P < 0.05.