miR-7 suppresses glioma cellsgrowth and glycometabolismin vitro. (A) The expression levels of miR-7 were determined by qRT-PCR in bothU87 cells and U251 cells. (B, C) Colony formation assay was used to detect the colony formation activity. (D, E) The glycolytic activity and maximum glycolytic capacity was determined in real-time using the Seahorse extracellular flux analyzer. ECAR were continuously calculated for two hours. ECAR following the addition of glucose defines glycolysis and ECAR following oligomycin represents maximum glycolytic capacity. Each data point represents the mean ± SD of three experiments. *p <0.05, **p <0.01.