Figure 1

Quantification of breast cancer biomarkers in tissues treated by decalcification: The study involved 9 cases and 5 fields per slide were measured at each time point. A - C) The average staining intensities at each time point across all slides are plotted on the Y-axis for the estrogen receptor (A), progesterone receptor (B) and p53 (C) and the % Ki-67 positive cells are platted in D. The X-axis indicates the time interval of the decalcification treatment. *p < 0.005 versus baseline. E) The %CV across 5 fields in each slide is shown for 29 slides stained for ER, 20 stained for PR, 12 stained for p53 and 18 stained for Ki-67.