Figure 3

Visualization of staining categories of Her2 membrane expression. A) Each point represents one region from a slide. Data of weak, moderate and strong positive cells and of negative cells were collected. Three categories (1, 2 and 3) of positive cells were defined based on t₁ and t₂ thresholds in Figure 2. The percentages of cells in weak, moderate and strong staining categories are plotted on X, Y and Z axes. Note clusters of HER2 grade 0 and 1+ (green and red dots), HER2 grade 2+ (black) and 3+ (pink). B) Relationship between staining score (mean +/- std) and clinical HER2 expression. Staining scores were calculated by summing up the percentages of cells multiplied by 1, 2 and 3.