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Table 1 Primers and LNA probes for LNA assay with Sanger sequencing confirmation

From: Validation of a locked nucleic acid based wild-type blocking PCR for the detection of EGFR exon 18/19 mutations

Exon

Name

Target

Amplicon size

Sequence (5’–3’)

18

18 FP

G719A/S/C

112 bp

CAGGAAACAGCTATGACCTGGAGCCTCTTACACCCAGT

18 RP

  

ACCGTGCCGAACGCACCGGA

18 LNA

  

GAGCCCAGC

19

19 FP

Deletions

176 bp

GTTAAAATTCCCGTCGCTATCA

19 RP

  

TGTAAAACGACGGCCAGTTGTGGAGATGAGCAGGGTCT

19 LNA

  

CAAGGAATTAAGA

20

20 FP

Insertions

161 bp

GAAGCCTACGTGATGGCCA

20 RP

  

CAGGAAACAGCTATGACCAGCAGGTACTGGGAGCCAAT

20 LNA

  

CAGCGTGGACA

-

M13-tag 1

For Sanger sequencing

-

TGTAAAACGACGGCCAGT

-

M13-tag 2

For Sanger sequencing

-

CAGGAAACAGCTATGACC

  1. Italic: M13-sequencing tag; F: forward primer; R: reverse primer; LNA: locked nucleic acid; bp: base pairs