Fig. 1

Immunohistochemically visualized expression of ALCAM in cutaneous melanoma. Lack of ALCAM immunoreactivity in melanoma cells from primary tumor (a, 200×, hematoxylin). Cytoplasmic distribution of ALCAM in melanoma cells from primary tumor (b, IRS 6, 400×, hematoxylin). High cytoplasmic ALCAM reactivity in primary melanoma (c, IRS 12, 400×, hematoxylin). Predominantly membranous-cytoplasmic expression of ALCAM in different sizes of nests composed of malignant melanoma cells from primary tumors (d, 400×, hematoxylin). Immunohistochemical pattern of ALCAM expression in regional lymph nodes metastases. Low ALCAM immunoreactivity in metastatic melanoma cells (e, 400×, hematoxylin). High cytoplasmic reactivity of ALCAM in regional lymph node metastases (f, 200×, hematoxylin)