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Table 3 Immunohistochemistry characteristics of positive samples and correlation with pyrosequencing mutational status. Strong / weak describes the intensity of staining and 1+ to 4+ referrers to the percentage of stained tumor cells. WT indicates wild-type samples according to pyrosequencing data

From: NRAS Q61R, BRAF V600E immunohistochemistry: a concomitant tool for mutation screening in melanomas

NRAS Q61 pyrosequencing NRAS Q61R Non NRAS Q61R
IHC NRASQ61R (SP174) Strong Weak Strong Weak
4+ 10 3 0 0
3+ 4 1 0 0
2+ 2 1 0 1 (WT)
1+ 1 1 0 0
BRAF V600 pyrosequencing BRAF V600E Non BRAF V600E
IHC BRAFV600E (VE1) Strong Weak Strong Weak
4+ 13 4 1 (WT) 2 (WT)
3+ 3 1 0 1 (WT)
2+ 0 0 0 0
1+ 0 2 0 1 (WT)