Table 3 Immunohistochemistry characteristics of positive samples and correlation with pyrosequencing mutational status. Strong / weak describes the intensity of staining and 1+ to 4+ referrers to the percentage of stained tumor cells. WT indicates wild-type samples according to pyrosequencing data
From: NRAS Q61R, BRAF V600E immunohistochemistry: a concomitant tool for mutation screening in melanomas
NRAS Q61 pyrosequencing | NRAS Q61R | Non NRAS Q61R | ||
|---|---|---|---|---|
IHC NRASQ61R (SP174) | Strong | Weak | Strong | Weak |
4+ | 10 | 3 | 0 | 0 |
3+ | 4 | 1 | 0 | 0 |
2+ | 2 | 1 | 0 | 1 (WT) |
1+ | 1 | 1 | 0 | 0 |
BRAF V600 pyrosequencing | BRAF V600E | Non BRAF V600E | ||
IHC BRAFV600E (VE1) | Strong | Weak | Strong | Weak |
4+ | 13 | 4 | 1 (WT) | 2 (WT) |
3+ | 3 | 1 | 0 | 1 (WT) |
2+ | 0 | 0 | 0 | 0 |
1+ | 0 | 2 | 0 | 1 (WT) |