Fig. 1

Differences in the CRT expression levels between hepatic cells and HCC cells and the effects of siRNA on CRT expression. a. Whole cell lysate from indicated HCC cells and L02 hepatic cells were subjected to Western blot analysis. β-actin served as a loading control. b. The expression of CRT in SMMC7721 and HepG2 cells was detected by Western blot analysis after transfected with CRT siRNA and negative control siRNA for 36 h. c. SMMC7721 cells were transfected with CRT siRNA and negative control siRNA for 36 h. The expression of CRT was detected by immunofluorescence. SMMC7721 without any transfection served as a negative control. d. HepG2 cells were transfected with CRT siRNA and negative control siRNA for 36 h. The expression of CRT was detected by immunofluorescence. HepG2 without any transfection served as a negative control. Figures shown are representatives of at least 3 independent experiments