Fig. 4
Down-regulation of CRT induces cell cycle progression arrest in HCC cells. a. SMMC7721 cells were transfected with CRT siRNA and negative control siRNA for 36Â h. Cell cycle progression was analyzed by propidium iodide staining method using flow cytometry. SMMC7721 without any transfection served as a negative control. The percentages of SMMC7721 cells in each phase were quantified. b. HepG2 cells were transfected with CRT siRNA and negative control siRNA for 36Â h. Cell cycle progression was analyzed by propidium iodide staining method using flow cytometry. HepG2 without any transfection served as a negative control. The percentages of HepG2 cells in each phase were quantified. Figures shown are representatives of at least 3 independent experiments. Statistical differences were compared with the siCtrl and NC (*P <0.05)