On the representation of cells in bone marrow pathology by a scalar field: propagation through serial sections, co-localization and spatial interaction analysis

Weis, Cleo-Aron; Grießmann, Benedict Walter; Scharff, Christoph; Detzner, Caecilia; Pfister, Eva; Marx, Alexander; Zoellner, Frank Gerrit

doi:10.1186/s13000-015-0383-0

Table 1 Comparison of direct and indirect interaction for three registered sections in a case with loose lymphoid infiltrates

From: On the representation of cells in bone marrow pathology by a scalar field: propagation through serial sections, co-localization and spatial interaction analysis

	Direct interaction/RBF_direct								Indirect interaction/RBF_indirect
	CD3	CD3 Bone	CD4	CD4 Bone	CD8	CD8 Bone	CD20	CD20 Bone	CD3	CD3 Bone	CD4	CD4 Bone	CD8	CD8 Bone	CD20	CD20 Bone
CD3		M_CD3 = 0.00	M_CD3 = 0.00^b	M_CD3 = 0.02	M_CD3 = 0.00^b	M_CD3 = 0.02	M_CD3 = 0.10^a	M_CD3 = 0.00		M_CD3 = 0.60	M_CD3 = 0.54	M_CD3 = 0.36	M_CD3 = 0.54	M_CD3 = 0.36	M_CD3 = 0.69^a	M_CD3 = 0.60
CD3 Bone	M_CD3Bone = 0.00		M_CD3Bone = 0.03	M_CD3Bone = 0.43^a	M_CD3Bone = 0.00	M_CD3Bone = 0.43^b	M_CD3Bone=0.00	M_CD3Bone=0.88	M_CD3Bone = 0.89		M_CD3Bone = 0.71	M_CD3Bone = 0.50	M_CD3Bone = 0.71	M_CD3Bone = 0.50	M_CD3Bone=0.59	M_CD3Bone=0.98
CD4	M_CD4 = 0.00^b	M_CD4 = 0.61		M_CD4 = 0.00	M_CD4 = 0.00	M_CD4 = 0.00	M_CD4 = 0.00	M_CD4 = 0.39	M_CD4 = 0.91	M_CD4 = 0.80		M_CD4 = 0.24	M_CD4 = 0.72	M_CD4 = 0.21	M_CD4 = 0.69	M_CD4 = 0.24
CD4 Bone	M_CD4Bone = 0.00	M_CD4Bone = 0.79^a	M_CD4Bone = 0.00		M_CD4Bone = 0.00	M_CD4Bone=0.84	M_CD4Bone=0.01	M_CD4Bone=0.31	M_CD4Bone = 0.88	M_CD4Bone = 0.83	M_CD4Bone = 0.36		M_CD4Bone = 0.66	M_CD4Bone=0.92	M_CD4Bone=0.54	M_CD4Bone=0.82
CD8	M_CD8 = 0.00^b	M_CD8 = 0.00	M_{CD8 =}0.00	M_CD8 = 0.00		M_CD8 = 0.00	M_CD8 = 0.00	M_CD8 = 0.00	M_CD8 = 0.91	M_CD8 = 0.80	M_{CD8 =} 0.50	M_CD8 = 0.31		M_CD8 = 0.27	M_CD8 = 0.80	M_CD8 = 0.51
CD8 Bone	M_CD8Bone = 0.00	M_CD8Bone = 0.71^b	M_CD8Bone = 0.00	M_CD8Bone = 0.96	M_CD8Bone = 0.00		M_CD8Bone=0.01	M_CD8Bone=0.25	M_CD8Bone = 0.89	M_CD8Bone = 0.83	M_CD8Bone = 0.21	M_CD8Bone = 1.00	M_CD8Bone = 0.64		M_CD8Bone=0.51	M_CD8Bone=0.83
CD20	M_CD20=0.15 ^a	M_CD20=0.00	M_CD20 = 0.00	M_CD20 = 0.06	M_CD20 = 0.00	M_CD20 = 0.07		M_CD20 = 0.00	M_CD20=0.99 ^a	M_CD20=0.57	M_CD20 = 0.59	M_CD20 = 0.31	M_CD20 = 0.99	M_CD20 = 0.27		M_CD20 = 0.58
CD20 Bone	M_{CD20Bone=0.00}	M_{CD20Bone=0.91}	M_CD20Bone=0.03	M_CD20Bone=0.46	M_CD20Bone=0.00	M_CD20Bone=0.44	M_CD20Bone=0.00		M_{CD20Bone=0.90}	M_{CD20Bone=0.99}	M_CD20Bone=0.36	M_CD20Bone=0.50	M_CD20Bone=0.68	M_CD20Bone=0.46	M_CD20Bone=0.60

Three sequential sections stained for CD3, CD20, CD4 and CD8 were registered and subsequently the overlap coefficients were calculated for direct and indirect interaction (application of RBF_direct with its sharp shape and respectively RBF_indirect with its broad silhouette)
^aAs expected, small overlap/direct interaction between CD3+ and CD20+ cells and noticeable indirect, paracrine interaction
^bUnexpected low overlap between the T cell markers CD3, CD4 and CD8 assumedly due to morphological changes in the course of serial sectioning (see arrows in Additional file 3: Figure S3). Modification of the RBF_direct would overcome this problem, however, at the cost of reduced specificity

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ISSN: 1746-1596

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