Table 1 Immunohistochemistry
Antibody | Clone | Manufacturer | Dilution | Pretreatment | Incubation with primary antibody | Visualisation kit manufacturer | Visualisation kit |
|---|---|---|---|---|---|---|---|
CD56 (NCAM) | 1B6 | Novocastra, UK | 1:50 | microwave oven, 20′, citrate buffer, pH6 | 1 h | Thermo Scientific™, USA | TL-125-HL |
Cytokeratin 19 | RCK108 | Dako, Denmark a/s | 1:100 | enzime digestion, preteinase K, 10′, room temp | 30′ | Thermo Scientific™, USA | TL-125-HL |
HBME-1 | HBME-1 | Dako,Denmark A/S | 1:50 | microwave oven, 20′, citrate buffer, pH6 | 1 h | Thermo Scientific™, USA | TL-125-HL |
Human Galecin-3 | 194804 | Techne/r&d systems, USA | 1:100 | microwave oven, 20′, citrate buffer, pH6 | 12 h, 4 °C | Thermo Scientific™, USA | TL-125-HL |