Hepatocellular adenoma classification: a comparative evaluation of immunohistochemistry and targeted mutational analysis

Table 2 Histological, immunohistochemical and mutational analysis of hepatocellular adenomas

	Morphological features		Immunohistochemistry				Mutational Analysis	Initial Classification^b	Final Classification^c
Case	Morphology	Atypical features	SAA	LFABP	B-cat	GS	Mutational Analysis	Initial Classification^b	Final Classification^c
1	0	NuA, Lret	1	2	0	2	CTNNB1 c.114_119del; p.38_40del¹ TP53 c.T751A; Ile251Phe^1,2	b-HCA	b-HCA
2	T	NuA, AG, Lret	1	2	1	2	IL6ST c.C657A; Pro216His^1,2,3	b-HCA	IHCA
3	T	0	0	2	1	2	IL6ST c.554_568del; p.185_190del^1,3	b-HCA	IHCA
4	0	NuA, AG, Lret	0	2	1	2	nm**	b-HCA	favor b-HCA
5^a	0	AG, Lret	1	2	0	1	n/a	IHCA	n/a
6	T, I	NuA, AG, Lret	1	2	0	1	nm**	IHCA	IHCA
7	T, I, DR	NuA	2	2	0	1	CTNNB1 c.T133C; Ser45Pro^1,3 IL6ST c.566_577del; p.189_193del^1,3	IHCA	bIHCA
8	T, I	0	2	2	0	0	nm**	IHCA	IHCA
9	T, I, DR	0	2	2	0	0	nm**	IHCA	IHCA
10	T, I, DR	0	2	2	0	1	nm**	IHCA	IHCA
11	T, DR	NuA, Lret	2	2	n/a	0	nm	IHCA	IHCA
12	T	0	2	2	0	0	n/a	IHCA	n/a
13^a	T, I, S (20 %)	0	2	2	0	1	nm**	IHCA	IHCA
14	T, I, DR, S (60 %)	0	2	2	n/a	0	nm**	IHCA	IHCA
15^a	T, I, DR	0	2	2	0	0	n/a	IHCA	n/a
16	S (30 %)	NuA	2	0	0	2	HNF1A c.620G > A; Gly207Asp^1,3	H-HCA	H-HCA
17	S (20 %)	0	2	0	0	1	HNF1A c.857A > G;Tyr286Cys¹	H-HCA	H-HCA
18	S (40 %)	0	0	0	0	0	HNF1A c.842 T > A;Leu281Gln¹	H-HCA	H-HCA
19	S (70 %)	0	0	0	0	0	n/a	H-HCA	n/a
20	S (80 %)	0	0	0	0	0	HNF1A c.710A > G; Asn237Ser^1,3	H-HCA	H-HCA
21	S (70 %)	0	2	0	0	0	HNF1A c.710A > T; Asn237Lys^1,3	H-HCA	H-HCA
22	S (10 %)	0	2	0	n/a	0	n/a	H-HCA	n/a
23	I, S (30 %)	0	2	0	0	0	n/a	H-HCA	n/a
24	T, I, S (50 %)	0	0	0	0	0	n/a	H-HCA	n/a
25^a	S (10 %)	0	0	2	0	1	n/a	UHCA	n/a
26	I	0	0	2	0	0	nm**	UHCA	UHCA

Abbreviations: B-HCA β-catenin activated hepatic adenoma, IHCA inflammatory HCA, bIHCA HCA with mixed IHCA and b-HCA features, H-HCA HNF1A-inactivated HCA, UHCA unclassifiable HCA, SAA serum amyloid A, LFABP liver fatty acid-binding protein, b-cat β-catenin, GS glutamine synthetase, 0 absent, T telangiectasia, I inflammation, DR ductular reaction, S Steatosis (% fat), NuA nuclear atypia, AG acinar growth, Lret focal loss of reticulin stain, n/a not available, nm no mutation/deletion/insertion identified in HNF1A and CTNNB1 exon 3, nm** no mutation/deletion/insertion identified in HNF1A, IL6ST, STAT3, GNAS, JAK1 and CTNNB (expanded gene analysis)
^aOnly biopsy specimen available; ^bClassification based on IHC alone; ^cClassification following molecular evaluation (italic font is used to mark cases that were re-classified after molecular analysis); 1Deleterious alteration by PROVEAN prediction; 2 variant previously described in HCC; 3 variant previously described in HCA

ISSN: 1746-1596