Automated quantification of proliferation with automated hot-spot selection in phosphohistone H3/MART1 dual-stained stage I/II melanoma

Table 4 Multivariate Cox regression analysis including Breslow thickness (continuous, log-transformed) and ulceration

PHH3 quantification type with index categorized by median	Progression-free survival
PHH3 quantification type with index categorized by median	HR	95 % CI	P
Manual count & outline, hot spot, 0 vs. ≥ 1.0	5.5	1.3 - 24	0.024
Manual count & outline, MART1-adjusted hot spot, ≤ 7.7/mm² vs. > 7.7/mm²	3.0	1.2 - 7.1	0.015
Manual count & outline, MART1-adjusted global, ≤ 3.3/mm² vs. > 3.3/mm²	1.9	0.82 - 4.3	0.14
Automated count & manual outline, hot spot, 0 vs. ≥ 1.0	1.9	0.92 - 3.8	0.085
Automated count & outline, hot spot, ≤ 2.0 vs. > 2.0	1.3	0.61 - 2.9	0.47
Automated count & outline, MART1-adjusted hot spot, ≤ 14/mm² vs. > 14/mm²	1.7	0.81 - 3.7	0.16
Automated count & manual outline, MART1-adjusted global, ≤ 4.0/mm² vs. > 4.0/mm²	1.3	0.62 - 2.7	0.49

NOTE. Only patients with a classified Breslow thickness were included (n = 144). Thus, the number of events was reduced to 39.
Abbreviations: PHH3 phosphohistone H3, HR hazard ratio, CI confidence interval

ISSN: 1746-1596