Development of a programmed cell death ligand-1 immunohistochemical assay validated for analysis of non-small cell lung cancer and head and neck squamous cell carcinoma

Table 1 Recommended staining protocol for VENTANA PD-L1 (SP263) Assay (OptiView DAB IHC kit, BenchMark ULTRA instrument)

Procedure type	Method
Deparaffinization	Selected
Cell conditioning (Antigen unmasking)	Cell conditioning 1, 64 min
Pre-primary peroxidase inhibitor	Selected
Antibody (primary)	16 min, 36 °C
OptiView HQ linker	8 min (default)
OptiView HRP multimer	8 min (default)
Counterstain	Hematoxylin II, 4 min
Post counterstain	Bluing, 4 min

DAB 3, 3’-diaminobenzidine tetrahydrochloride, HRP horse radish peroxidase, HQ hydroxyquinoxaline, IHC immunohistochemistry, PD-L1 programmed cell death ligand-1

ISSN: 1746-1596