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Table 1 Recommended staining protocol for VENTANA PD-L1 (SP263) Assay (OptiView DAB IHC kit, BenchMark ULTRA instrument)

From: Development of a programmed cell death ligand-1 immunohistochemical assay validated for analysis of non-small cell lung cancer and head and neck squamous cell carcinoma

Procedure type Method
Deparaffinization Selected
Cell conditioning (Antigen unmasking) Cell conditioning 1, 64 min
Pre-primary peroxidase inhibitor Selected
Antibody (primary) 16 min, 36 °C
OptiView HQ linker 8 min (default)
OptiView HRP multimer 8 min (default)
Counterstain Hematoxylin II, 4 min
Post counterstain Bluing, 4 min
  1. DAB 3, 3’-diaminobenzidine tetrahydrochloride, HRP horse radish peroxidase, HQ hydroxyquinoxaline, IHC immunohistochemistry, PD-L1 programmed cell death ligand-1