Table 1 Sociodemographic. clinical. morphological. immunohistochemical and cytogenetic data of the diagnostic groups after FISH
Diagnostic Groups after FISH | ||||||
|---|---|---|---|---|---|---|
BL (n = 15) | DHL (n = 3) | DLBCL (n = 72) | ||||
n | % | n | % | n | % | |
Sociodemographic data | ||||||
Woman | 6 | 40 | 2 | 66.7 | 29 | 40.3 |
Man | 9 | 60 | 1 | 33.3 | 43 | 59.7 |
Age over 60 years old | 0 | 0 | 3 | 100 | 34 | 47.2 |
HIV | 1 | 6.7 | 0 | 0 | 2 | 2.8 |
LNH history | 0 | 0 | 0 | 0 | 2 | 2.8 |
Viral hepatitis | 0 | 0 | 0 | 0 | 4 | 5.5 |
Morphology | ||||||
Monomorphic | 15 | 100 | 3 | 100 | 51 | 70.8 |
Polymorphic | 0 | 0 | 0 | 0 | 21 | 29.1 |
“Starry sky” pattern | 14 | 93.3 | 0 | 0 | 17 | 23.6 |
Diffuse growth | 15 | 100 | 3 | 100 | 71 | 98.6 |
Necrosis | 1 | 6.7 | 1 | 33.3 | 26 | 36.1 |
Mitotic activity | 12 | 80 | 1 | 33.3 | 52 | 72.2 |
Apoptosis | 14 | 93.3 | 3 | 100 | 57 | 79.1 |
Vesicular nuclear pattern | 4 | 26.7 | 1 | 33.3 | 52 | 72.2 |
Evident nucleolus | 5 | 33.3 | 0 | 0 | 64 | 88.8 |
Lobed nucleous | 2 | 13.3 | 0 | 0 | 30 | 41.6 |
Dense chromatin | 10 | 66.7 | 3 | 100 | 18 | 25 |
Loose chromatin | 6 | 40 | 0 | 0 | 54 | 75 |
Endothelial proliferation | 4 | 26.7 | 1 | 33.3 | 35 | 48.6 |
Cytoplasmic eosinophilia | 7 | 46.7 | 3 | 100 | 43 | 59.7 |
Soft tissue infiltration | 4 | 26.7 | 1 | 33.3 | 38 | 52.7 |
Stromal sclerosis | 0 | 0 | 0 | 0 | 19 | 26.3 |
Immunohistochemistry | ||||||
CD20 | 15 | 100 | 3 | 100 | 68/70 | 97.1 |
CD79a | 14 | 93.3 | 2 | 66.7 | 66/70 | 94.3 |
PAX5 | 11 | 73.3 | 1 | 33.3 | 49/70 | 70 |
CD10 | 15 | 100 | 3 | 100 | 31/71 | 43.6 |
Bcl6 | 7 | 46.7 | 2 | 100 | 22/70 | 31.4 |
Bcl2 | 0 | 0 | 2 | 100 | 29/71 | 40.8 |
MUM1 | 0 | 0 | 0 | 0 | 37/71 | 52.1 |
TDT | 0 | 0 | 0 | 0 | 0 | 0 |
Myc | 5 | 33.3 | 0 | 0 | 11/70 | 15.7 |
Ki-67 > 90% | 3 | 20 | 2 | 66.7 | 9/70 | 12.8 |
Cytogenetic | ||||||
MYC | 15 | 100 | 3 | 100 | 3 | 4.2 |
BCL2 | 0 | 0 | 2 | 66.7 | 11 | 15.3 |
BCL6 | 0 | 0 | 1 | 33.3 | 9 | 12.5 |
Prognostic markers | ||||||
High levels of LDH | 5/6 | 83.3 | 2 | 66.7 | 37/47 | 78.7 |
Staging III/IV | 9 | 60 | 0 | 0 | 29/71 | 40.8 |
Bone marrow infiltration | 2 | 13.3 | 0 | 0 | 12/71 | 16.9 |
CNS infiltration | 1 | 6.7 | 0 | 0 | 4/71 | 5.6 |
B symptoms | 9 | 60 | 1 | 33.3 | 31/71 | 43.6 |
Extranodal involvement | 8 | 53.3 | 2 | 66.7 | 35/71 | 49.3 |
Nodal involvement | 8 | 53.3 | 1 | 33.3 | 44/71 | 61.9 |
Therapeutic data | ||||||
Chemotherapy | 11 | 73.3 | 2 | 66.7 | 65/71 | 91.5 |
Chemotherapy and radiotherapy | 1 | 6.6 | 0 | 0 | 17 | 26.6 |
Bone marrow transplant | 0 | 0 | 0 | 0 | 1 | 1.4 |
Death | 5 | 33.3 | 3 | 100 | 30/66 | 45.4 |
Recurrence | 0 | 0 | 0 | 0 | 2/59 | 3.4 |