Fig. 5

Comparison of frozen sections (upper row) and FFPE sections (lower row) of control muscle (left column) and muscle biopsy from patient 13 (right column). All samples were immunostained with an antibody against the N-terminus of dystrophin. A diagnosis of BMD was established because of the absence of staining for the N-terminus of dystrophin in the frozen section (b) and positive staining for the N-terminus of dystrophin in control tissue (a), while other sarcolemmal-membrane associated proteins were strongly expressed (data not shown). The FFPE muscle of the patient (d) could not be interpreted because of unsuccessful sarcolemmal staining of the control muscle (c). Original magnification, ×400