Table 1 Antibodies used for immunohistochemistry
Target | Clone | Company | Dilution | Incubation |
|---|---|---|---|---|
CDX2 | EPR2764Y | CellMarque | 1:400 | 15 min |
CK20 | Ks 20.8 | CellMarque | 1:800 | 15 min |
CK7 | OV-TL 12/30 | CellMarque | 1.800 | 15 min |
Oestrogen | EP1 | DAKO | 1:50 | 30 min |
MLH1 | ES05 | Novocastra | 1:200 | 15 min |
MSH2 | G219-1129 | CellMarque | 1:500 | 15 min |
MSH6 | PU29 | Novocastra | 1:100 | 15 min |
p16 | E6H4 | Ventana | 1:5 | 15 min |
Pax8 | polyclonal | Proteintech | 1:200 | 15 min |
PMS2 | A16-4 | BD Pharmingen | 1:100 | 15 min |
Progesterone | 16+ SAN27 | Novocastra | 1:1600 | 30 min |
WT1 | 6 F-H2 | CellMarque | 1:100 | 15 min |
AFP | polyclonal | DAKO | 1:1600 | 15 min |
SALL4 | 6E3 | Biocare Medical | 1:400 | 15 min |
Gypican 3 | 1G12 | CellMarque | 1:200 | 30 min |