Fig. 1

Overview of DecisionDx-UM testing and analytic validation. Fine-needle aspirate biopsies are collected prior to plaque or proton beam clip placement, snap-frozen in RNA stabilization buffer, and shipped frozen. Alternatively, six 5-μm section slides containing tumor tissue from enucleations can be used. RNA is extracted and reverse transcribed into cDNA. The cDNA is pre-amplified followed by qPCR for 12 discriminating genes and 3 control genes. A support vector machine (SVM) algorithm assigns a Class 1 or 2 call comparing the patient sample to a locked-down training set. The summation of CDH1 and RAB31 is used to determine Class 1A or 1B subclassification