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Fig. 1 | Diagnostic Pathology

Fig. 1

From: Data integration from pathology slides for quantitative imaging of multiple cell types within the tumor immune cell infiltrate

Fig. 1

Visualizing individual antibody combinations before and after color separation in digital images. (a-d) Representative of invasive breast cancer stained with Pan-Cytokeratin (Pan-CK) and CD45. The tissue section was stained using an IHC protocol with 2 antibodies. The stained slide was imaged using the Aperio slide scanner and the colors were deconvoluted. (a) Original image of 2-antibody RGB stain, (b) Pan-CK+ breast cancer cells (red), (c) CD45+ lymphocytes (brown), and (d) hematoxylin positive nuclei (blue). (e-h) Representative image of tumor area parallel to (a) and stained with 3 antibodies. The stained slide was imaged using the Vectra-II slide scanner and the colors were unmixed. (e) Original RGB image of 3-antibody stain, (f) CD4+ cells (brown), (g) CD8+ lymphocytes (purple), and (h) CD68+ macrophages (red)

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Diagnostic Pathology

ISSN: 1746-1596

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