Fig. 3
Correlation between intra- and extra-tumoral immune infiltrate. Tumor areas were outlined by hand in images from 81 cases. (a) CD45+ cells were counted in slides stained for CD45 and Pan-CK, after regional separation of intra-tumoral, tumor border and extra-tumoral regions. The y-axis represents cell counts per mm2 outside the tumor area, while the x-axis depicts cell counts within tumor nest plus cell counts at the border. The Pearson’s correlation coefficient is shown. (b) CD4+, (c) CD8+, and (d) CD68+ immune cell numbers were obtained from slides stained with the 3-antibody IHC protocol and analyzed as described in (a). Pearson’s correlation coefficients are shown for concordance of TIL subtypes inside and outside nests of tumor cells