Fig. 3

MYC immunohistochemistry and fluorescent in situ hybridization (FISH). a Large atypical plasmablasts were c-MYC positive in >40% of cells. b FISH analysis using Vysis MYC Break Apart FISH Probe Kit revealed 8q24 rearrangement within tumour cells (cells without rearrangement should have 2 yellow fusion signals; magnification × 1000). c FISH analysis using Vysis LSI IGH/MYC, CEP 8 Tri-color Dual Fusion Translocation Probe is negative for t(8,14), without yellow signals specific for IGH/MYC translocation (normal 2 green (IgH) and 2 red (MYC, 8q24) signals); magnification × 1000