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Table 5 Mutation detection in ctDNA by real-time PCR and MS approaches

From: Molecular profiling of lung cancer specimens and liquid biopsies using MALDI-TOF mass spectrometry

Patients with EGFR/KRAS mutated tumors Mutation in FFPE tumors (mutated allele percentage) Mutation detection in ctDNA by real-time PCR (ΔCt values) Mutation detection in ctDNA by MS (mutated allele percentage)
1 EGFR L858R (56%) D (2.55) EGFR L858R (7%)
2 EGFR E746_A750delELREA (27%) D (1.44) EGFR E746_A750delELREA (5%)
3 EGFR E746_A750delELREA (60%) D (3.66) EGFR E746_A750delELREA (12%)
4 EGFR E746_A750delELREA (55%) D (3) EGFR E746_A750delELREA (19%)
5 EGFR E746_E750delELREA (50%) D (4.02) ND
6 EGFR E746_A750delELREA (30%) ND (12.61) ND
7 EGFR E746_A750delELREA (30%) ND (14.03) ND
8 EGFR L858R (18%) ND (18.99) ND
9 KRAS G12 V (50%) ND (27.23) ND
10 KRAS G12 V (41%) ND (18.78) ND
11 KRAS G12C (17%) ND (19.63) ND
12 KRAS G12 V (33%) ND (27.23) ND
13 KRAS G12C (20%) ND (16.95) ND
  1. D: detected. ND: not detected