Fig. 3From: Does heterogeneity matter in the estimation of tumour budding and tumour stroma ratio in colon cancer?How to distinguish fibrous tumour stroma from smooth muscle tissue. a In the H&E stained section it is challenging to distinguish between fibrous stroma and smooth muscle tissue. b Immunohistochemical stain for desmin highlights the smooth muscle tissue. c Masson trichrome highlights the collagen fibres in the fibrous stroma (blue) and the smooth muscle tissue (purple-red). Magnification 10×Back to article page