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Fig. 2 | Diagnostic Pathology

Fig. 2

From: A case report of granulomatous amoebic encephalitis by Group 1 Acanthamoeba genotype T18 diagnosed by the combination of morphological examination and genetic analysis

Fig. 2

Histopathological findings of brain biopsy sample. a and b Hematoxylin and eosin staining with low (a) and medium magnification (b) of the brain biopsy sample. Necrotic tissue with inflammatory cell infiltration and vessel hyalinization was observed. Scale bar: 100 μm (for a) and 20 μm (for b). c, d, e, f and g Hematoxylin and eosin staining of amoebic cysts (c) and trophozoites (d) in the brain biopsy sample. These pathogens were observed in necrotic tissues and cysts were also observed around blood vessels (e). f shows the magnified image of square area in (e). These cysts showed faint positive in Periodic acid-Schiff stain (g, arrows). Scale bar: 10 μm (for c and d), 50 μm (for e) and 20 μm (for f and g). h and i Immunohistochemical staining of amoebic cysts. The amoebic cysts were positive for antiserum against Acanthamoeba (h), but negative for Balamuthia (i). Scale bar: 20 μm

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