Fig. 2

Characterization of the mAbs against HPV18 L1-VLP. a The binding strength of mAbs 1B1 and 4C2 to HPV18 L1-VLPs. OD₄₅₀ is the optical density at 450 nm; the concentrations of the mAbs are given in μg/ml. The reaction curves were sharply increased at the turning point of 0.05 μg/ml. The results indicate that both 1B1 and 4C2 mAbs have similar binding capabilities to HPV18 L1-VLPs; b and c Specificities of mAbs 1B1 and 4C2 to native or denatured HPV L1-VLPs. The optical density at 450 nm (OD₄₅₀) of mAbs 1B1, 4C2 mAbs were plotted on the y-axis against various genotypes of native or denatured VLPs on the x-axis. The bar graph was prepared using Prism GraphPad 6.0. All mAb samples were adjusted to an initial concentration of 0.3 μg/ml. All values are the average of multiple holes. L is the native protein, and D is the denatured protein