Fig. 2
From: PD1hi cells associate with clusters of proliferating B-cells in marginal zone lymphoma
Architecture of CD4+ T-cell infiltration and T-cell gene signatures. a Tissue sections from 6 cases were stained with anti-CD20, anti-CD4 and anti-CD8. Surface plots generated in ImageJ (https://imagej.net/Fiji) are shown for each stain. Intensity has been normalised so that images can be compared. T-cell zones are evident and whilst these comprised both CD4 and CD8 T-cells, CD4 T-cells were found at higher density in 4 of 6 cases (#1, #2, #4 and #6). b Gene set enrichment analysis. Data from Arribas et al. (2012)5 was employed to discover T-cell signatures. Utilising a false discovery rate (FDR < 0.25) for exploratory work. Two gene signatures were found to be positively enriched in MZL. NES is normalised enrichment score. c Support for use of PD1hi and FOXP3+ as single surrogate markers of Tfh and Treg cells respectively. Numbers of Tfh and Treg cells, as defined (Fig. 1d), were compared against single marker expression of PD1hi and FOXP3