Fig. 3

Variation of ALK immunoreactivity in a series of 12 lung adenocarcinomas. Three representative cases of microfluidic-based ALK IF (MTP IF ALK) are shown in a Case N°10, b Case N°7 and c Case N°3. Scale bars are 100 μm and 10 μm for the first and second column, respectively. The third column represents the negative control (CTL NEG) where PBST 0.05% was used instead of the primary antibody. The shown region of interest for the negative control is the same as for ALK IF; scale bar is 10 μm. d Quantification of the ALK IF signals. For each case, 25 ROIs were manually placed in both ALK+ tumor epithelia and stroma regions. The graph shows the average of the mean intensities of all 25 ROIs for tumor epithelia (blue) and stroma (orange) with corresponding standard deviations. Case N°11 is present twice (11#1 and 11#2) as two samples with distinct localization (lung primary and parietal pleura, respectively) were obtained from the same patient