Fig. 2
From: Dual monoclonal antibody-based sandwich ELISA for detection of in vitro packaged Ebola virus
Characterization of the mAbs 6E3 and 3F21. a. Western blot showed that 6E3 and 3F21 recognized GP1 and GP2, respectively. b. Dot-blot results showed that 3F21 could recognize 1 ng GP protein, whereas 6E3 could only recognize 10 ng GP protein per dot. c. Molecular interaction analysis between GP and mAbs 3F21 and 6E3 showed that the affinity of 3F21 towards GP was stronger than that of 6E3 at the same antibody concentration. d. Neutralizing capacities of 6E3 and 3F21 were evaluated by EBOV pseudovirus. 4G7 served as a neutralizing mAb positive control. 5G10 acted as an irrelative antibody control