Fig. 2

Circ_0003998 knockdown mitigates DOX resistance in HCC cells. Huh7/DOX and HCCLM3/DOX cells were transfected with si-circ_0003998 or si-NC. After transfection, a qRT-PCR analysis of circ_0003998 expression; b, c CCK-8 analysis of resistant cell viability in combination with increasing concentrations of DOX (0, 0.0625, 0.125, 0.25, 0.5, or 1 μg/mL); d colony formation analysis of resistant cell viability with 0.5 μg/mL DOX; e, f transwell analysis of the abilities of migration and invasion in resistant cells; g, h western blot analysis of MMP9, Vimentin, N-cadherin, and E-cadherin levels in resistant cells. *P < 0.05