Fig. 4

Circ_0003998 knockdown sensitizes HCC cell to DOX by sponging miR-218-5p. Huh7/DOX and HCCLM3/DOX cells were transfected with si-NC, si-circ_0003998, si-circ_0003998 + anti-miR-NC, or si-circ_0003998 + anti-miR-218-5p. After transfection, a qRT-PCR analysis of miR-218-5p expression; b-d the viability analysis of resistant cell in combination with DOX using CCK-8 assay and colony formation analysis; e, f migration and invasion analysis of resistant cells with transwell assay; g, h levels analysis of MMP9, Vimentin, N-cadherin, and E-cadherin in resistant cells using western blot. *P < 0.05