Table 1 Antibodies employed in the immunohistochemical study and results
Growth pattern | ||||||
|---|---|---|---|---|---|---|
Antibody | Acinar-glandular pattern | Solid pattern | Overlying gastric mucosa | Clone | Source | Dilution |
BCL-10 | +++ | ++ | – | 331.3 | Santa Cruz Biotechnology | 1:50 |
Trypsin | +++ | ++ | – | Merck Millipore | 1:2000 | |
Chromogranin A | + | +++ | – | SP12 | Thermo Fisher Scientific | 1:75 |
Synaptophysin | + | ++ | – | SP11 | Thermo Fisher Scientific | 1:75 |
CD56 | + | ++ | – | Nichirei Bioscience | prediluted | |
Amylase | + | + | + | |||
Insulin | – | – | – | DakoCytomation | 1:100 | |
Glucagon | – | – | – | DakoCytomation | 1:200 | |
Gastrin | – | – | – | DakoCytomation | 1:300 | |
Serotonin | – | – | – | DakoCytomation | 1:50 | |
Somatostatin | – | – | – | Roche | prediluted | |
Pancreatic polypeptide | – | – | – | DakoCytomation | 1:600 | |
β-catenin | + (cell membrane, cytoplasm) | DakoCytomation | 1:200 | |||
CK7 | – | – | + | OV-TL12/30 | DakoCytomation | 1:75 |
CK19 | – | – | + | RCK108 | DakoCytomation | 1:75 |
CK20 | – | – | + | KS20.8 | DakoCytomation | 1:75 |
CAM5.2 | + | + | + | Becton, Dickinson and Company | prediluted | |
CKAE1/AE3 | + | + | + | DakoCytomation | prediluted | |