A comparative study of next-generation sequencing and fragment analysis for the detection and allelic ratio determination of FLT3 internal tandem duplication

Table 2 Concordance of FLT3 ITD mutation burden classification between NGS and fragment analysis

Fragment analysis	Next-generation sequencing		Total	% Agreement
Fragment analysis	Low mutation burden	High mutation burden	Total	% Agreement
Low mutation burden	30	0	30 (46.9%)	54.7%
High mutation burden	29	5	34 (53.1%)
Total	59 (92.2%)	5 (7.8%)	64

ISSN: 1746-1596